Pengaruh Perioperative Albumin Infusion dan Diet Normal Protein terhadap Perubahan Sitokin Proinflamsi (TNFα, IL1 and IL6) dan CR

Utariani ., E. Raharjo, D.S. Perdanakusuma

Abstract


Abstrak

Malnutrisi dapat mempengaruhi sitokin proinflamasi TNF, IL1, IL6 dan CRP perioperatif, sehingga dapat meningkatkan morbiditas dan mortalitas. Sedangkan patofisiologi interaksi ini masih belum jelas. Penelitian ini dirancang untuk menganalisis peran perioperatif albumin infus dan protein diet pada perubahan sitokine proinflamasi TNFa, IL1, IL6 dan CRP. Penelitian eksperimental murni dilakukan dengan menggunakan dua puluh lima tikus Sprague Dawley diacak dan dibagi menjadi 5 kelompok; kelompok kontrol (A) yang diberi diet protein normal, kelompok hipoalbuminemia diberikan infus albumin praoperasi (B), kelompok hipoalbuminemia dengan perioperatif diet protein normal (C), kelompok hipoalbuminemia diberiinfus albumin pasca operasi (D) dan hypoabuminemia dengan diet rendah protein (E). Metode pemeriksaan Elisa digunakan untuk mengukur plasma TNF, IL1, IL6, dan CRP. Penelitian ini menunjukkan hasil diet protein rendah praoperasimeningkatkan TNF, IL1, IL6 dan CRP secara signifikan. Sedangkan pemberian infus albumin dan diet protein normal praoperasi menurunkan TNF, IL1, IL6 dan CRP secara signifikan, pemberian infus albumin praoperasi dan pasca operasi terjadi perubahan penurunan TNF, IL1, IL6, dan CRP namun tidak signifikan. Simpulan penelitian ini adalah pemberian infus Albumin dan diet protein normal menurunkan sitokin proinflamasi (TNF , IL1 , IL6), dan CRP secara signifikan. Hal ini juga berarti dapat menurunkan morbiditas dan mortalitas .

Kata kunci: Diet protein, perioperatif albumin, sitokin proinflamasi

The Effect of Perioperative Albumine Infussion and Normal Protein Diet on Proinlfammatory Cytokine (TNFα, IL1,IL6) and CRP

Abstract

Protein malnutrition may affect perioperative TNFα, IL1 ,IL6 and CRP,that increases morbidity and mortality. The pathophysiology of this interaction is still unclear. This study was designed to analyze the role of perioperative albumin infusion and protein diet on the changes of TNFα, IL1, IL6 and CRP. The laboratory experimental research with post test only-control group design was conducted. Twenty five Sprague Dawley Rats were randomized and divided into 5 groups; control group which was given normal protein diet, hypoalbuminemia group given preoperative albumin infusion, hypoalbuminemia with perioperative normal protein diet, hypoalbuminemia group given postoperative albumin infusion and hypoabuminemia with low protein diet. Four incisions were made on the rats for operation model. Elisa method was used to measure plasma TNFα, IL1, IL6 and CRP. This study showed that as the result of preoperative low protein diet, TNFα, IL1, IL6 and CRP increased significantly. The albumin infusion and normal protein diet then decreased the TNFα, IL1 and CRP significantly, whereas the changes were not significant in preoperative and postoperative albumin infusion. conclution from this research is Albumin infusion and normal protein diet significantly decrease proinflammatory cytokines (TNFα, IL1, IL6) and CRP on hypoalbuminemia state. It can be suggested that they also decrease the morbidity and mortality.

Key words: Perioperative albumin, proinflammatory cytokines, protein diet


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